Journal: American Journal of Cancer Research

Article Title: MLL5α activates AR/NDRG1 signaling to suppress prostate cancer progression

doi:

Figure Lengend Snippet: Overexpression of MLL5α promotes PCa cell sensitivity to ENZ treatment. (A and B) C4-2 (Aa and Ba) and PC3 (Ab and Bb) oe-MLL5α/NC cells were treated with 20 μM ENZ or the same volume of DMSO. A CCK-8 assay (A) and colony formation assay (B) were performed. The results are presented as the means ± SEMs. *P < 0.05. (C-F) Mice xenografted with C42-oe-MLL5α/NC cells were treated with 25 mg/kg/day ENZ or the same volume of DMSO and were randomly divided into four groups: oeNC-DMSO, oeMLL5α-DMSO, oeNC-ENZ, and oeMLL5α-ENZ. (C) Photographs of tumors on the fiftieth day after the inoculation of cells into the axilla of mice. (D) Tumor growth curves (a) and excised tumor weights (b) were quantitatively analyzed. The results are presented as the means ± SEMs. *P < 0.05, **P < 0.01 and ***P < 0.001. (E) Representative images of IHC staining for cleaved caspase-3 (C-Casp-3); Ki-67; TUNEL; and HE staining in each group. (F) Quantitative results of the staining percentage (a) and staining index (b) in each group. The results are presented as the means ± SEMs. ns P > 0.05, *P < 0.05, and **P < 0.01.

Article Snippet: Histological and immunohistochemical (IHC) analyses Histological and IHC analyses were performed by Wuhan Servicebio Technology (Wuhan, China).

Techniques: Over Expression, CCK-8 Assay, Colony Assay, Immunohistochemistry, TUNEL Assay, Staining

Journal: American Journal of Cancer Research

Article Title: MLL5α activates AR/NDRG1 signaling to suppress prostate cancer progression

doi:

Figure Lengend Snippet: Low expression of MLL5α in advanced stages of PCa. PCa tissues were divided into 4 groups: normal tissue, GS ≤ 6, GS = 7, and GS ≥ 8. A. Representative images (a) of IHC for MLL5α in the four groups of PCa patients. Quantitative results of the staining percentage (b) and staining index (c) in each group. The results are presented as the means ± SEMs. *P < 0.05. B. Relative mRNA levels (normalized to those in adjacent normal tissues) of MLL5α in 15 PCa patients (a) and relative mRNA levels (normalized to those of 18S ribosomal RNA) in the four groups of PCa patients (b). The results are presented as the means ± SEMs. *P < 0.05 and **P < 0.01. C. MLL5α protein levels as determined via WB analysis in PCa patients compared to those in adjacent normal tissues (a) and MLL5α protein levels in the GS ≥ 8 group compared to those in the GS ≤ 6 group (b).

Article Snippet: Histological and immunohistochemical (IHC) analyses Histological and IHC analyses were performed by Wuhan Servicebio Technology (Wuhan, China).

Techniques: Expressing, Staining

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: Characteristics tested tissue specimens of patients with invasive breast cancer with Leica HER2 FISH.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Staining

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: “Pictures”: at least 4 areas of interest are selected for analysis and pictures are taken automatically. Captured images from different focus planes are combined generating a picture with all signals clearly detectable after removal of unspecific background. “Objectives and tools”: automated nucleus selection and spot counting of the red ( HER2 ) and the green (chromosoom 17 = CEP17 ) signal is evaluated and corrected by the observer if needed. “Analysis”: supervised-automated scoring is performed on at least two pictures and the ratio between HER2 and CEP17 was generated.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Selection, Generated

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis in 20 full-sized slides of tissue specimens used for TMA blocks of invasive breast cancer.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Amplification

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis in tissue micro arrays (TMAs) including 328 invasive breast cancer tissue specimens.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Amplification

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: Representative pictures of HER2 non-amplified invasive breast cancer specimens are shown in A) with a HER2 (red signals) to chromosoom 17 (= CEP17 ; green signals) ratio <2 and C) with supervised-automated nuclei and spot detection. Examples of HER2 amplified invasive breast cancer specimens are depicted in B) with a HER2 / CEP17 ratio >2 and D) with supervised-automated nucleus and spot detection. DAPI counterstaining and original magnification with 100x objective.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Amplification

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: Comparisons of automated Leica HER2 FISH with digital analysis and manual Abbott HER2 FISH with manual analysis of 50 invasive breast cancer tissue specimens (resection / biopsy) with IHC 2+ scores.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Amplification

Journal: PLoS ONE

Article Title: Fully Automated Fluorescent in situ Hybridization (FISH) Staining and Digital Analysis of HER2 in Breast Cancer: A Validation Study

doi: 10.1371/journal.pone.0123201

Figure Lengend Snippet: Comparisons of Leica HER2 FISH and DAKO HER2 IHC of 50 consecutively collected invasive breast cancer tissue specimens (resection / biopsy) from routine practice.

Article Snippet: The HER2 gene status determined by the available Visia imaging D-Sight HER2 FISH analysis module (software version 2011 1.3.15) is based on the FISH scoring criteria according to ASCO guideline 2007 [ ] as described in the previous section.

Techniques: Amplification

Journal: OncoTargets and therapy

Article Title: Genes Regulated by HPV 16 E6 and High Expression of NFX1-123 in Cervical Cancers

doi: 10.2147/OTT.S251926

Figure Lengend Snippet: NFX1-123 and proliferation and differentiation marker protein expression in HPV16 positive cervical cancer tissues. ( A ) Immunohistochemical staining of NFX1-123, Notch1, Ki67, and Keratin 1 in HPV16 positive cervical tumors (n = 34). Representative images of high, medium, and low staining are shown for each protein. Number of tumors and corresponding percent of total tumors graded as high, medium, or low staining were quantified and shown in graphs (right). ( B ) Heat map representation of the NFX1-123, Notch1, Ki67 and Keratin 1 expression in cervical cancer samples (n = 34) and normal cervical epithelial samples (n = 31). The cervical tumors are clustered by histologic classification (carcinoma in situ (CIS), moderately differentiated, poorly differentiated, or invasive squamous cell carcinomas (SCC)) and the normal cervical tissues clustered by cytologic classification (negative or reactive cellular changes). The staining intensity of both the HPV 16 positive cervical cancers and the normal cervical specimens were converted to a four-point colorimetric scale: 1= non-detected, 2 = low, 3 = medium, 4 = high.

Article Snippet: Supporting this, 100% of the cervical tumors in the Human Protein Atlas database had medium to high expression of NFX1 (n=11) ( ).

Techniques: Marker, Expressing, Immunohistochemical staining, Staining, In Situ

Journal: OncoTargets and therapy

Article Title: Genes Regulated by HPV 16 E6 and High Expression of NFX1-123 in Cervical Cancers

doi: 10.2147/OTT.S251926

Figure Lengend Snippet: NFX1 and NFX1L1 mRNA and protein expression was upregulated in cervical and head and neck cancers. ( A ) mRNA expression of NFX1 and NFX1L1 in normal cervix (n = 10) and cervical primary tumors (n = 303) from TCGA Target GTEx database were plotted for graphical presentation using GraphPad Prism. ( B ) IHC analysis of NFX1 protein expression in normal cervical tissues (n = 3) and cervical tumors (n = 11) from The Human Protein Atlas database. Representative images of normal cervical tissue and cervical tumors are shown. M = medium staining intensity, H = high. Number of tumors and corresponding percent of total tumors graded as high or medium were quantified and shown in graphs (right).

Article Snippet: Supporting this, 100% of the cervical tumors in the Human Protein Atlas database had medium to high expression of NFX1 (n=11) ( ).

Techniques: Expressing, Staining

Journal: OncoTargets and therapy

Article Title: Genes Regulated by HPV 16 E6 and High Expression of NFX1-123 in Cervical Cancers

doi: 10.2147/OTT.S251926

Figure Lengend Snippet: mRNA expression of genes regulated by HPV 16E6 and NFX1-123 were significantly upregulated in cervical cancer. mRNA expression of 19 genes regulated by 16E6 and NFX1-123 were analyzed using TCGA Target TGEx in normal cervix (n = 10) compared to cervical primary tumors (n = 303). Significance was determined using two-tailed Mann–Whitney test. 14 of 19 genes were significantly upregulated in cervical tumors compared to normal tissue. LOR, CEBPD, LCE2B, FOXA 2, and FBN2 were deregulated in cervical cancers, although they did not reach statistical significance. Note the Y-axis scales for individual gene expression datasets were adjusted to demonstrate samples with no detectable gene expression (0 value).

Article Snippet: Supporting this, 100% of the cervical tumors in the Human Protein Atlas database had medium to high expression of NFX1 (n=11) ( ).

Techniques: Expressing, Two Tailed Test, MANN-WHITNEY, Gene Expression

Journal: OncoTargets and therapy

Article Title: Genes Regulated by HPV 16 E6 and High Expression of NFX1-123 in Cervical Cancers

doi: 10.2147/OTT.S251926

Figure Lengend Snippet: HPV 16E6/NFX1-123 regulated proteins were differentially expressed in cervical cancers. Relative expression of nine 16E6/NFX1-123 regulated proteins were compared in cervical tumors and normal cervical samples from The Human Protein Atlas database. Representative images of the IHC staining for the 9 genes are shown for normal cervix (left) or for cervical tumors (middle panels). Number of tumors and corresponding percent of total tumors graded as high, medium, or low staining were quantified and shown in graphs (right). Abbreviations: ND, not detected; L, low; M, medium; H, high.

Article Snippet: Supporting this, 100% of the cervical tumors in the Human Protein Atlas database had medium to high expression of NFX1 (n=11) ( ).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: OncoTargets and therapy

Article Title: Genes Regulated by HPV 16 E6 and High Expression of NFX1-123 in Cervical Cancers

doi: 10.2147/OTT.S251926

Figure Lengend Snippet: Protein expression of genes upregulated with overexpressed NFX1-123 and 16E6 in HPV positive cell lines. Western blot analysis of NFX1-123 and 16E6 regulated proteins ( A ) RAET1G and Notch1 ( B ) TGM1 and ( C ) FOXA2 in primary human foreskin keratinocytes (HFK), HPV 16 positive cervical cancer cell lines (SiHa and CaSki), and a HPV 18 positive cervical cancer cell line (HeLa). Beta-actin was used as a loading control. Fold expression of each protein of interest is shown for these cervical cancer cell lines relative to typical expression in HFKs.

Article Snippet: Supporting this, 100% of the cervical tumors in the Human Protein Atlas database had medium to high expression of NFX1 (n=11) ( ).

Techniques: Expressing, Western Blot, Control